Abstract: A rapid evaluation method for avian influenza oil emulsion vaccine antigen based on isopropyl myristate as a demulsifier was established，which is low cost and environmentally friendly，and the experimental conditions are simple. It can accurately and quickly determine the HA titer of finished oil emulsion vaccines such as avian influenza and analyse their antigenic differences within a few hours. The method can facilitate the selection of high quality and efficient vaccines from different sources and the determination of their antigenic potency before use， avoiding the use of low potency vaccines and ensuring the expected control effect.

Key words: Avian influenza vaccine； Antigenic quality； Evaluation method

摘要： 本研究建立了基于肉豆蔻酸异丙酯作为破乳剂的一种禽流感油乳剂疫苗抗原快速 检测方法，该方法成本低且环境友好，实验条件简易，保真度高，可在数小时内准确快速测定 禽流感等油乳剂疫苗成品抗原HA效价及分析其抗原性差异。使用该方法能够方便广大养殖 用户从不同来源疫苗中有针对性地选择优质高效的疫苗，并在使用疫苗前确定其抗原效价， 避免使用低效价疫苗，确保预期防控效果，同时也方便兽药监察部门对市场上各个厂家的禽 流感油乳剂灭活疫苗的质量进行实时监测。

关键词: 禽流感疫苗； 抗原质量； 评价方法