Abstract: The purpose of this experiment was to study the effect of Newcastle Disease Virus（NDV）on the migration of peripheral blood T lymphocytes in different geneotypes of Yellow ? feathered chickens，and to provide theoretical knowledge for exploring the immune response mechanism of chickens to NDV. Using Transwell co?culture system，the upper chamber and the lower chamber were cultured with lymphocytes and dendritic cells，respectively. After NDV challenging，the lymphocyte suspension was collected at 0，2，4，6 h of each time point. The total T lymphocyte migration rate was obtained by the flow cytometry method and the optimal co ? cultivation time wasdetermined. Then the migration rate of total T lymphocytes in wild?type（TT），hybrid?type（CT）and homozygotic?type （CC）were compared after NDV challenge. The results showed that the migration rate of total T lymphocytes was 45.04% after 2 hours of NDV challenge. In the groups challenged by virus，the migration rate of total T lymphocytes in CT genotype was 60.03% ，and the total T lymphocytes migration rate in TT genotype was 45.40% . There was a significant difference between the two genotypes（P<0.05）. However，there was no significant difference between CT and CC genotype（P>0.05）. In conclusion，the time of optimal lymphocytes culture was 2 hours，which was suitable for cell migration. After NDV challenge，the migration rate of total T lymphocytes in CT genotype was faster than that of TT genotype.

Key words: chickens； Newcastle disease virus； T lymphocytes； Cell migration

摘要： 本试验研究新城疫病毒（Newcastle disease virus，NDV）对鸡外周血T淋巴细胞迁移 的影响，为探讨鸡对NDV免疫应答机制提供理论支撑。采用Transwell 共培养，上室为淋巴细 胞，下室为树突状细胞（Dendritic cells，DC），通过NDV攻毒刺激后，分别在第0、2、4、6 h收集 各个时间点上室淋巴细胞悬液，用流式细胞法计数得出总T淋巴细胞迁移率，确定淋巴细胞与 树突状细胞迁移共培养时间模型为2 h。进一步分析NDV对野生型（TT）、杂合型（CT）和纯合型 （CC）的三种岭南黄羽肉鸡的外周血总T淋巴细胞迁移率的影响。结果表明：树突状细胞攻毒 刺激2 h后的淋巴细胞迁移率为45.04%；在攻毒组中，CT基因型鸡淋巴细胞的总T淋巴细胞迁 移率最高为60.03%，TT基因型鸡淋巴细胞的总T淋巴细胞迁移率最低为45.40%，两组间差异 显著（P<0.05）；而CT和CC基因型之间没有差异（P>0.05）。综上所述，淋巴细胞迁移培养时间 点为2 h比较适合；NDV攻毒后CT基因型的T淋巴细胞迁移率显著高于TT基因型。

关键词: 鸡； 新城疫病毒； T淋巴细胞； 细胞迁移