Table of Content

18 December 2025, Volume 50 Issue 6

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Research Advances in the Nutrition of Energy, Protein and Amino Acids of Broilers in 2024#br#

QI Zhihao, JIANG Shouqun, LAN Ruixia, ZHANG Sai

2025, 50(6):  1-5.  DOI: 10.19978/j.cnki.xmsy.2025.06.01

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In 2024，the research on the nutrition of energy，protein，and amino acids in broilers focused on precise nutritional regulation and sustainable development， aiming to optimize feed efficiency， production performance，and intestinal health. Studies have shown that the energy requirements of broilers are affected by multiple factors such as genetics，growth stage，environment，and feed composition. Reasonable adjustment of the energy density can significantly improve growth performance and feed conversion rate. The technology of low⁃protein diets continues to develop. Through the balance of amino acids and the supplementation of synthetic amino acids，it can maintain production performance while reducing nitrogen emissions. The precise supply of essential amino acids such as lysine and methionine is the key. The role of functional amino acids in immune regulation and intestinal health has been deeply explored，and the interaction mechanism between them and intestinal microorganisms has become a research hotspot.

Research Progress on Prevention Treatment of Porcine Viral Infectious Diseases with Traditional Chinese Medicine#br#

MA Chunxia, ZHANG Yang, LIU Xueshuang, LI Cuiyun, WU Ya, PAN Qin, REN Shaoke, OU Yunwen

2025, 50(6):  6-13.  DOI: 10.19978/j.cnki.xmsy.2025.06.02

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Abstract：In recent years，with the rapid development of China 's pig industry. With the expansion of breeding and transportation scale，porcine reproductive and respiratory syndrome virus （PRRSV），porcine circovirus 2 （PCV2），porcine pseudorabies virus（PRV）and porcine epidemic diarrhea virus（PEDV）are becoming more and more prevalent. It has caused huge economic losses to the pig industry. As a traditional Chinese medicine，traditional Chinese medicine has a long history of use. It has the effects of antiviral，anti⁃inflammatory and enhancing immunity. These effects together constitute the cornerstone of the core strategy of traditional Chinese medicine for the treatment of viral infections. With the deepening of related research，traditional Chinese medicine has achieved remarkable results in the prevention and treatment of PRRSV，PCV2，PRV and PEDV，and has broad application prospects. Therefore，this paper reviews the application of traditional Chinese medicine in the prevention and treatment of porcine viral infectious diseases，in order to provide reference for the prevention and treatment of porcine viral infectious diseases and the development of new antiviral Chinese veterinary drugs.

Anti⁃nutritional Effect of Soybean Antigenic Protein on Aquatic Animals and Its Mitigation Measures#br#

TIAN Renhong, PENG Kai, DONG Ruiqi, LU Huijie, LIU Zhenxing, HUANG Wen

2025, 50(6):  14-19.  DOI: 10.19978/j.cnki.xmsy.2025.06.03

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Soybean antigenic proteins represent the primary allergens triggering allergic reactions in animals. Upon ingestion，these proteins compromise the intestinal antioxidant capacity of aquatic species，damage intestinal tissue structure，induce inflammatory response，and impair mucosal barrier function. Consequently，they suppress growth and development in aquatic animals，thereby limiting the application of soybean protein source in compound feeds for aquaculture. This review systematically examines the antinutritional effects of soybean antigenic proteins on aquatic animals and discusses potential mitigation strategies. The findings provide a theoretical support for enhancing the utilization efficiency of soybean protein，optimizing compound formulations，and reducing feed costs in aquatic animal production.

Epidemic Status, Transmission Mechanism and Prevention and Control Strategies of Chikungunya Fever

QIAN Yanhong, ZHANG Pian, CHEN Jing, ZHANG Xiaoxiao, WANG Xiaohu

2025, 50(6):  20-28.  DOI: 10.19978/j.cnki.xmsy.2025.06.04

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Chikungunya fever is an acute mosquito⁃borne infectious disease caused by the Chikungunya virus（CHIKV） and transmitted primarily by Aedes mosquitoes. The main clinical manifestations include fever，rash，and joint and muscle pain. The disease exhibits distinct epidemiological features and urban transmission cycles，posing a high infection risk to human populations. Factors such as viral genetic mutations，accelerated globalization，and climate warming have driven the global spread of chikungunya fever in recent years. To date，chikungunya outbreaks have been reported in 119 countries and regions，with an estimated annual cumulative case count exceeding 500 000. Due to the absence of approved commercial vaccines or effective antiviral therapies，Chikungunya fever continues to pose a significant threat to human health，public health systems，and socioeconomic development. Currently，the global spread of chikungunya fever is becoming increasingly severe. Notably，the 2025 outbreak in Foshan，Guangdong􀆼linked to imported cases and subsequent local transmission􀆼 has underscored the urgent need for enhanced prevention and control measures in China. This paper systematically reviews the global and domestic epidemiological status of chikungunya fever，its transmission routes and local transmission characteristics，underlying mechanisms and influencing factors，as well as comprehensive prevention and control strategies. The study aims to provide valuable insights for the scientific prevention and control of epidemic importation and spread in China and for safeguarding public health.

Analysis of Pathogenic Factors and Comprehensive Prevention and Control Measures of Salpingitis in Laying Hens

WANG Shenglin, HUO Xinghua, SUN Jing, LI Yanfang, CAO Xiuqin, CHEN Haijun, CHEN Shan, Qian Huiwen

2025, 50(6):  29-34.  DOI: 10.19978/j.cnki.xmsy.2025.06.05

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Salpingitis is a common multiple disease during the laying period，which can lead to a decline in the laying rate，with symptoms including the absence of an laying peak period，deterioration in eggshell quality，and a decrease in egg quality. The etiological factors contributing to salpingitis are complex，mainly associated with pathogenic bacterial infections， inappropriate feeding management， nutritional deficiencies， substandard environmental hygiene，and disease prevention and control strategies. Starting from the hazards of salpingitis，this article outlines the potential pathogenic factors，and proposes comprehensive measures that need to be taken in the production management process of laying hens，in order to provide a reference for peers in the laying hen industry to effectively prevent and control salpingitis.

Cross⁃species Transmission Mechanisms of Streptococcus suis Influenced by Climate and Geographical Factors

WU Hanguang, LI Yingxin, CHEN Yuqing, SHI Yuanguo, LI Fan, LI Wenxuan, ZHOU Jianzhuo, KE Yankun

2025, 50(6):  35-40.  DOI: 10.19978/j.cnki.xmsy.2025.06.06

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Streptococcus suis (S. suis) is a significant zoonotic pathogen exhibiting distinct spatiotemporal distribution patterns in cross⁃species transmission. This review systematically examines how climate and geographical factors influence the transmission dynamics of S. suis. Through comprehensive analysis of global literature from 2000 to 2024，we demonstrate that：climate elements regulate both the seasonality and intensity of transmission risk by affecting pathogen survival in the environment，host immune status，and vector activity；geographical isolation shapes the genetic diversity and population structure of S. suis in different regions，forming genotype distribution patterns with distinct regional characteristics；Southeast Asia is a global transmission“hotspot”，where the combination of high temperature and humidity climate with foodborne transmission significantly increases human infection risk；serotype 2 S. suis shows obvious advantages in cross⁃species transmission with strong“serotype filtering”effects. Based on the climate⁃geography⁃pathogen ternary interaction mechanism，multi⁃factor risk assessment models and differentiated prevention strategies are proposed to provide theoretical basis for constructing scientific early warning systems and formulating precise control measures.

Comparative Study of Different Cryopreservation Systems for Chicken Primordial Germ Cells

SUN Shengrui, ZHU Ying, ZHAO Haoyi, HE Yanhua, ZHAO Zhifeng, LUO Chenglong, SHU Dingming, ZOU Xian

2025, 50(6):  41-48.  DOI: 10.19978/j.cnki.xmsy.2025.06.07

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To identify the optimal cryopreservation protocol for chicken primordial germ cells（PGCs），eight cryopreservation solutions — including both literature ⁃ reported and commercially available formulations—were evaluated. Two different cell concentrations were cryopreserved under identical culture conditions and processing method. Post⁃thaw analyses，including trypan blue exclusion assay，CCK⁃8 cell proliferation assay，and apoptosis detection were used to performed to assess cell viability and status in each group，thereby evaluating the performance of the eight cryoprotectants at each cell concentration. The results showed that t Cryoprotectant 7 yielded the highest number of viable cells at a concentration of 1.37×106 cells/mL，reaching 1.14×106，with 74.8% of cell being negative for both fluorescein isothiocyanate（FITC）and propidium iodide（PI）. In contrast，Cryoprotectant 1 yielded the highest number of viable cells at 2.75×106 cells/mL，reaching 2.60×106，with 75.2% ouble⁃negative cells . However， Cryoprotectants II and Ⅷ showed the least favorable outcomes. These findings indicate that the effectiveness of cryoprotectants varies with cell concentration. The most suitable solution for cryopreserving PGCs at 1.37×106 cells/mL in 250 μL was Solution Ⅶ（Saichang Biological Solution）. For cryopreservation solution PGCs at 2.75×106 cells/mL， the optimal formulation was SolutionⅠ（basal medium∶chicken serum∶DMSO∶CaCl2=81.25%∶10%∶8%∶0.75%）.

Establishment of in Vitro Culture System of Spermatogonial Stem Cells Isolated from Testis Samples of Guangdong Small⁃spotted Pigs

XIN Haiyun, ZHOU Shimeng, MENG Fanming

2025, 50(6):  49-54.  DOI: 10.19978/j.cnki.xmsy.2025.06.08

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The study aimed to study the characterization of spermatogonial stem cells（SSCs）from Guangdong Small⁃spotted Pig testis in vitro after separation，purification and in vitro culture. The investigation employed enzyme digestion， density gradient centrifugation， and differential adhesion methods for cell enrichment， and cell immunofluorescence staining was used to assess separation efficiency，cell proliferation and differentiation. The results indicated that the combination of three separation methods effectively improved the purity of SSCs，which were successfully proliferated in vitro，forming distinct cell colonies by day 14，then cell colonies proliferation was significantly increased by day 21. Immunostaining showed that the SSCs maker，UCHL⁃1 was expressed highly within 28 days，while the expression of C⁃kit on day 56 indicated cell differentiation.

Effects of Lactobacillus Supplemented in Drinking Water on Intestinal and Immune Organ of Broiler Breeders during the Late Laying Period#br#

WANG SanJun, LIN Chun, LIANG Yongkang, XU Xiayu, XU Bin, ZHANG Chang, MEI Gui, DU Zongliang, LI Ying

2025, 50(6):  55-60.  DOI: 10.19978/j.cnki.xmsy.2025.06.09

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This study investigated the effects of Lactobacillus supplementation in drinking water on the intestinal and immune organs in broiler breeders during the late laying period. The aim is to provide a theoretical basis for the application of Lactobacillus in poultry husbandry. A total of 360 healthy Silkie fowls（Black⁃boned chickens），aged 38 weeks with average weight of（2.4±0.1）kg，were randomly assigned to a control group and a treatment group，each with 6 replicates of 30 birds per replicate. The control group was provided withnormal drinking water，while the treatment group wasgiven a Lactobacillus nutrient solution（2 kg/t，with a viable bacterial count of 3×109 CFU/g） in their drinking water. Following a 7⁃day adaptation period，the experimental trial lasted 65 days. Results demonstrated that，compared to the control group，at day 30，the relative weight and organ index of the jejunum and cecumin the Lactobacillus ⁃ supplemented group were significantly increased （P<0.05）. At day 65，there was no significant difference in the intestinal organ index or relative weight between groups（P>0.05），but the thymus organ index was significantly increased（P<0.05），and other immune organ index showed a tendency for improvement. These findings indicate that adding Lactobacillus to drinking water can mitigate age⁃related decline of intestinal organs and enhance immune organ function in late⁃laying broiler breeders.

Comparative Analysis of the Immune Effects of Recombinant Avian Influenza Virus（H5+H7）Trivalent Inactivated Vaccine on Specific Pathogen-Free-Chickens and Shelduck

DONG Jiawen, ZHANG Yanan, HUANG Yunzhen, CHEN Lei, KUANG Ruihuan, ZENG Xiaoduo, XIE Lexin, SUN Minhua

2025, 50(6):  61-68.  DOI: 10.19978/j.cnki.xmsy.2025.06.10

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To investigate the differences in immune effects of recombinant avian influenza virus（H5 + H7） trivalent inactivated vaccine on poultry and waterfowl，this study immunized different groups of SPF chickens and shelduck with the recombinant avian influenza virus（H5+H7）trivalent inactivated vaccine at 14 days of age（primary immunization）and 42 days of age（secondary immunization），respectively. Blood samples were collected before and weekly after vaccination to detect avian influenza virus HI antibody titer against H5 Re⁃13、H5 Re⁃14 and H7 Re⁃4 . Additionally，the expression levels of cytokine IL ⁃ 2，IL ⁃ 4，and IL ⁃ 6 in the serum 14 days after the second immunization（56 days of age）were assessed. The results showed that all three commercially available H5 + H7 trivalent inactivated vaccines could induce high HI antibody levels in SPF chickens within four weeks of primary immunization，with 100% qualification rates for H5⁃Re13，H5⁃Re14，and H7⁃Re4 antibodies. However，after primary immunization in shelduck，the qualification rate for H5⁃Re13 antibody was only 60%，with a low average titer，while the qualification rates for H5⁃Re14 and H7⁃Re4 antibodies were 60%⁃100% and 90%⁃100%，respectively. SPF chickens and shelduck could further increase HI antibody levels after booster immunization，but the antibody levels decreased four weeks after booster immunization. The immunogenicity of H5 subtype avian influenza virus inactivated vaccine in shelduck was much lower than that in chickens，while the H7 subtype avian influenza virus inactivated vaccine had high immunogenicity in both chickens and shelduck. The recombinant AIV trivalent inactivated vaccine did not induce the expression of cellular immune cytokines（IL⁃2 and IL⁃4）or significantly induce the expression of humoral immune cytokine IL ⁃ 6 in SPF chickens and shelduck. There was no direct correlation between the the differences in HI antibody levels induced by the various vaccines and the expression levels of cytokines IL⁃2，IL⁃4， and IL⁃6.

Comparative Analysis of Morphology Characteristics，Growth and Reproductive Performance among Yueyang Pigeon，Shiqi Pigeon#br# and the Mimas Strain of European Meat Pigeon#br#

XU Dong, GUO Shiqi, XU Kunjie, CHEN Yitian, WU ZhongPing, FANG Quanmin, YU Lian, LIANG Yayan, ZHANG Xumeng

2025, 50(6):  69-78.  DOI: 10.19978/j.cnki.xmsy.2025.06.11

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As China's meat pigeon industry has become the fourth largest poultry sector following chickens， ducks，and geese，the Yueyang pigeon，a unique genetic resource of Yueyang County in Hunan Province，boasts an annual slaughter output of over 10 million birds. However，there is a lack of systematic research on its breed characteristics. To evaluate the differences between Yueyang pigeon and other breeds，this study conducted a comparative analysis of the morphology traits，growth performance，and reproductive performance of Yueyang pigeon， Shiqi pigeon，and the European meat pigeon Mimas strain. The results revealed significant differences in body shape， head shape，legs，and feather color among the three breeds. Regarding growth performance，the Mimas strain exhibited extremely significant advantages in body weight，shank circumference，and chest depth over the other two breeds（P<0.01）. The body slant length of Yueyang pigeon was extremely significantly greater than that of the other two（P<0.01）. In male pigeons，the Mimas strain showed extremely significantly greater shank length and chest width than the other breeds（P<0.01），and its neck circumference was extremely significantly larger than that of Yueyang pigeon（P<0.01）. In terms of slaughter performance，the Mimas strain had the highest live body weight at slaughter， and its semi⁃evisceration yield was extremely significantly higher than that of Yueyang pigeon（P<0.01）. The full⁃ evisceration yield of Yueyang pigeon was significantly higher than that of the other two breeds（P<0.05），and its abdominal fat percentage was extremely significantly higher than that of Shiqi pigeon（P<0.01）. For reproductive performance，the number of clutches and the number of chicks hatched of Shiqi pigeon were extremely significantly lower than those of the Mimas strain（P<0.01），but the annual squab production of both Shiqi and Mimas was extremely significantly higher than that of Yueyang pigeon（P<0.01）. In meat and egg quality，Shiqi and Mimas pigeons were superior in egg weight and pH value，while Yueyang pigeon demonstrated better performance in meat redness（a* value），shear force，and yolk color. In conclusion，significant or extremely significant differences were observed in growth performance，reproductive performance，and meat and egg quality among the three pigeon breeds. This study clarifies the breed characteristics and production potential of Yueyang pigeon，providing data and theoretical references for the conservation of its germplasm resources，optimization of breeding programs，and high⁃ quality development of the industry.

Key Points for Breeding Technique of Large⁃scale Siniperca Chuatsi Seeding in Yangchun

CHEN Xiaoying, SUN Yuping, HUANG Wen, Ao Daojian, Huang Biao, Wu Zejin

2025, 50(6):  79-84.  DOI: 10.19978/j.cnki.xmsy.2025.06.12

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In recent years，the Siniperca chuatsi industry has witnessed a rapid expansion in scale，playing a key role in ensuring the stable supply of“vegetable basket”products and f improving fishery efficiency，and increasing fishermen's incomes. Moreover，it has become an important characteristic industry in some regions for promoting rural revitalization and strengthening the local economy. One of the key constraints on the development of the Siniperca chuatsi industry lies in the supply of high ⁃ quality seeds. Yangchun City is the predominant production area for mandarin fish seeds. The city places great emphasis on the development of the Siniperca chuatsi seed industry and has promoted the construction and operation of the Yangchun City Siniperca chuatsi seed modern agricultural industrial park. This paper summarizes the key aspects of large⁃scale Mandarin fish seed breeding technology in Yangchun municipal Siniperca chuatsi seed industrial park，aiming to provide reference for further research and promotion of large⁃scale Siniperca chuatsi seed breeding technology in Guangdong Province and across the country.

Cascade⁃cage Technique for Laying Duck Farming

RAO Dengming, XU Zhuangping, LUO Sangdanda

2025, 50(6):  85-90.  DOI: 10.19978/j.cnki.xmsy.2025.06.13

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With the rapid development of the livestock and poultry industry，traditional free⁃range farming is no longer adequate to meet the demand of large ⁃ scale and intensive production. The multi ⁃ tier cage system，as a innovative model for laying duck production，can effectively address key challenges in conventional farming，such as resource limitations，low production efficiency，environmental pressures，and difficulties in disease prevention and control. Based on practical production experience，this article summarizes key aspects of the multi⁃tier cage farming technology for laying ducks，including housing design，breed selection，feed nutrition，farm management，disease prevention，and benefit analysis. It aims to provide technical guidance and reference for laying duck producers.

Captive Husbandry and Breeding of Correlophus ciliatus

CAO JiaYue, CHEN ZhaoKun, ZHANG Lingna

2025, 50(6):  91-96.  DOI: 10.19978/j.cnki.xmsy.2025.06.14

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The crested gecko（Correlophus ciliatus）is an emerging specialty economic animal with a relatively short history in captive breeding but significant developmental potential. This review synthesizes critical technologies for its artificial husbandry and reproduction，aiming to provide a scientific foundation for large⁃scale farming. By analyzing habitat setup （e.g.， climbing structures， temperature/humidity control）， daily management （diet formulation，handling of shedding and tail autotomy handling），and breeding strategies（estrus detection，incubation optimization），this study systematically examines the characteristics and challenges of C. ciliatus aquaculture. Key findings demonstrate that habitat design must simulate arboreal niches，where container height，cork bark（e.g.， Portuguese cork wood），and humidity（70%-80%）are essential to prevent floppy tail syndrome as well as shedding complications. Reproductive success depends on breeder body weight（females ≥40 g，males ≥75 g）and incubation temperature（22.7-24.4℃），which significantly influence offspring sex ratio and viability. Disease management should prioritize calcium deficiency， gastrointestinal parasites， and environmental stressors （e.g.， head ⁃ shrinking syndrome）. In conclusions，Sustainable C. ciliatus farming necessitates balancing natural behavioral ecology with human intervention in captive environments. Despite rapidly growing market demand，scaling production faces challenges such as unstable hatching rates and insufficient genetic understanding of morphs traits. This review provides practical guidelines to enhance farming efficiency and animal welfare，offering actionable insights for advancing the reptile pet industry.

Prevention and Control of Brucellosis in Lianshan Black Goats

LIU Jingping

2025, 50(6):  97-99.  DOI: 10.19978/j.cnki.xmsy.2025.06.15

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Black goats are a traditional livestock and poultry breed in Lianshan Zhuang and Yao Autonomous County，Qingyuan City，mainly raised through traditional grazing and free range. They face high risk of disease spread and weak biosafety management in the prevention and control of brucellosis. This study aims to understand the difficulties in preventing and controlling brucellosis in black goats in Lianshan County through field investigations， and explore and develop scientific prevention and control strategies. The results showed that the proportion of free range households of Heishan sheep in Lianshan Zhuang and Yao Autonomous County was 96% . The climate characteristics of“cold winters and humid summers”in the local area，overlapping grazing routes，and traditional manure treatment methods under feeding patterns have increased the potential risk of brucellosis infection and transmission. Therefore，in the process of raising black goats，Lianshan Zhuang and Yao Autonomous County should adhere to self breeding and self raising，maintain a hygienic feeding environment，control the temperature of the sheep house and other prevention and control measures to ensure the healthy breeding of black goats.

Diagnosis and Treatment of Urinary Obstruction in a Neutered Male Cat

DONG Chunlin, YE Junjie, SONG Jia, LIU Shuyang

2025, 50(6):  100-105.  DOI: 10.19978/j.cnki.xmsy.2025.06.16

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This study aims to explore the diagnostic and therapeutic methods for feline urinary retention. As a common manifestation of Feline Lower Urinary Tract Disease（FLUTD），feline urinary retention can lead to systemic infection or even death in severe cases. In this case，feline urinary retention was initially suspected through inspection，inquiry，and palpation；further confirmed by imaging examinations including DR and abdominal color Doppler ultrasound；and finally diagnosed based on the results of blood routine，biochemical indicators，and coagulation function tests. After 3 days of conservative treatment，the cat's symptoms showed no significant improvement，so perineal urethrostomy was performed. The cat achieved good postoperative recovery and a favorable prognosis. This case indicates that for urinary retention cases unresponsive to conservative treatment，perineal urethrostomy can effectively improve the quality of life of affected cats，providing valuable reference for clinical practice.

Isolation，Identification and Drug Sensitivity Test of a Trueperella pyogenes Strain from Vulpes zerda

WANG Guoqian, CHEN Xuanjiao, ZENG Fanwen, SA Jiaqi, PENG Shiming

2025, 50(6):  106-111.  DOI: 10.19978/j.cnki.xmsy.2025.06.17

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To identify the pathogenic bacteria causing the death of a Vulpes zerda from respiratory failure at a zoo in Guangdong Province，lung tissue samples were collected for bacterial isolation and purification，morphological microscopy，biochemical tests，antimicrobial susceptibility testing，16S rRNA gene sequencing，phylogenetic tree construction and virulence gene detection. Results showed that one strain of Trueperella pyogenes（a Gram⁃positive， short⁃rod⁃shaped bacterium）was isolated from the lung tissue. Biochemical tests indicated that the isolate was positive for lactose，maltose，and glucose fermentation，but negative for sucrose，rhamnose，and mannitol utilization. 16S rRNA gene sequencing revealed more than 99% homology between the Vulpes zerda isolate and reference strains of T. pyogenes from swine and cattle，clustering within the same branch on the phylogenetic tree. Virulence gene detection confirmed the presence of at least six virulence genes：Plo ⁃ g2，Plo ⁃ g1，NanP，FimA，FimE，and FimC. Antimicrobial susceptibility testing demonstrated sensitivity to multiple antibiotics tested，with resistance observed only to two sulfonamide antibiotics（sulfamethoxazole and trimethoprim）. This study enriches the characterization of Trueperella pyogenes derived from Vulpes zerda，providing a reference for research on wildlife pathogens

A Case Report of Angiostrongylus cantonensis Infection in a Dog

CHEN Huayuan, CHEN Siyuan, LI Qing, GONG Mofeng, YU Jiezhou, LI Shaochuan

2025, 50(6):  112-115.  DOI: 10.19978/j.cnki.xmsy.2025.06.18

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Canine neurla angiostrongylosis （CNA） is a parasitic disease caused by the infection with Angiostrongyliasis cantonensis，characterized primarily by severe neurological dysfunction. This article discusses the diagnosis，prevention and control of CNA，aiming to provide a reference for clinical practice. A 5⁃year⁃old male Bichon Frise was presented with paralysis after reportedly ingesting a giant African land snail. Diagnostic investigations included clinical examination，complete blood count，cerebrospinal fluid（CSF）analysis，magnetic resonance imaging（MRI），and polymerase chain reaction（PCR）testing. CSF smear examination revealed elongated structures morphologically consistent with parasitic nematodes. MRI findings included T2 ⁃ weighted hyperintense signals in the thoracolumbar spinal cord and mild leptomeningeal enhancement in the brain. Based on these findings， the history of possible snail ingestion，and the endemic geographical location，a definitive diagnosis of A. cantonensis infection was confirmed. The dog died three days after presentation without receiving treatment. This case indicates that canine neural angiostrongylosis is rapidly progressive and carries a high mortality risk，underscoring the need for prompt clinical recognition and effective diagnostic and therapeutic interventions.

Teaching Reform in the Herbivore Feeding Science Courses: Innovative Approaches Integrating Ideological and Political Education， Practical Application，and Interdisciplinary Integration

WU Jiao, YUE Dan , CHONG Yuqing , DENG Weidong, WANG Bo, GAO Zhendong, WU Dongwu, XI Dongmei

2025, 50(6):  116-120.  DOI: 10.19978/j.cnki.xmsy.2025.06.19

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As a core course in Grassland Science，“Herbivore Feeding Science”holds significant importance in talent cultivation. However，traditional teaching models suffer from disconnections between theory and practice， monotonous teaching methods，and insufficient interdisciplinary integration. This paper，taking the curriculum reform at Yunnan Agricultural University as an example，explores a multifaceted integrated teaching approach：firstly， systematically incorporating ideological and political elements to cultivate students‘national pride，ecological civilization awareness，and professional responsibility；secondly，strengthening practical training and case analysis by establishing a three⁃tier progressive model of“basic experiments⁃comprehensive training⁃external practice”to enhance students’ability to solve real ⁃ world problems；thirdly，promoting interdisciplinary knowledge integration by combining feeding science with ecology，economics，food science，and information technology to broaden students ‘comprehensive literacy and innovative thinking. In a word，this teaching reform model effectively improves students’ professional skills，social responsibility，and interdisciplinary perspective，providing valuable insights for cultivating applied talents in animal husbandry.

Innovation and Practice of the Talent Cultivation Models in Animal Production through Deep Integration of Industry -Academia-#br# Research#br#

GAO Chunqi, XU Kengran

2025, 50(6):  121-124.  DOI: 10.19978/j.cnki.xmsy.2025.06.20

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The current talent cultivation system in animal production programs in higher education still faces structural contradictions，such as the disjunction between“education and industry”and the separation of“research from application，”leading to a misalignment between talent development and industry needs. This paper analyzes the new characteristics of talent demands in the animal husbandry industry and examines the bottlenecks in existing talent cultivation models. Using the construction of the Industry-Academia-Research Integrated Practical Teaching Base at South China Agricultural University as a case study，it elaborates on specific measures implemented by the base， including the establishment of a“3.5 + 0.5”education model，the development of dual - qualified teachers，the restructuring of the curriculum system，and the creation of shared practical platforms. The study aims to provide practical paradigms for deepening industry􀆼education integration and innovating talent cultivation mechanisms.