Abstract: To investigate the prevalence and drug resistance of Escherichia coli（E. coli）in a pigeon farm in Guangdong，E. coli was isolated and identified from the rectal contents of dead pigeons in this study，conducted antibiotic susceptibility tests on the isolates using agar dilution method，and also detected the drug resistance genes of the isolates using PCR. The results showed that 120 E. coli strains were isolated from 150 samples. The antibiotic susceptibility tests showed that the isolates were resistant to Ciprofloxacin，Ampicillin，and other antibiotics to varying degrees. Of the 120 isolates，74 strains had multiple drug resistance. The PCR detection revealed that the isolates with drug resistance phenotype carried 6 resistance genes，including qnrS、blaTEM、blaCTX⁃M、blaVIM 、aaC4 and sul1. These results indicated that the isolates are resistant to 13 antibiotics to varying degrees，with most of them having multiple resistance. Moreover，the isolates carry multiple resistance genes. This study could provide reference for the prevalence，antibiotic resistance status and clinical rational drug use of pigeon⁃source E. coli in Guangdong Province.

Key words: Pigeons, Escherichia coli（E. coli）, Isolation and identification, The prevalence, Drug resistance

摘要： 为调查广东某肉鸽场大肠杆菌（Escherichia coli，E. coli）的流行情况以及耐药性，该 研究从肉鸽直肠内容物中分离大肠杆菌并进行鉴定，采用琼脂稀释法对分离得到的大肠杆菌 进行药敏试验，通过PCR检测分离株携带的耐药基因。结果显示，150份样品共分离到120株 大肠杆菌；药敏试验结果显示分离株对环丙沙星、氨苄西林等13种抗菌药物不同程度耐药，其 中74 株大肠杆菌分离株存在多重耐药情况；PCR 检测发现具有耐药表型的菌株携带qnrS、 blaTEM、blaCTX?M、blaVIM、aaC4 和sul1 6 种耐药基因。上述结果表明，大肠杆菌分离株对13种抗菌 药物不同程度耐药，存在多重耐药现象，且携带多种耐药基因。该研究可为广东省鸽源大肠 杆菌的流行情况、耐药现状及临床合理用药提供参考。

关键词: 鸽, 大肠杆菌, 分离鉴定, 流行情况, 耐药性