Abstract: Abstract：Lipoprotein⁃associated phospholipase A2（Lp⁃PLA2），encoded by PLA2G7 gene，is mainly secreted by inflammatory cells and is associated with various metabolic and immune dysfunctions，as well as other related diseases. The sequence characteristics and physical and chemical properties of the protein encoded by PLA2G7 gene were analyzed to provide a theoretical basis for studying the mechanism of Lp ⁃ PLA2 protein in chicken growth， development and avian disease immunity. Here，bioinformatic methods were used to analyze the physical and chemical properties，hydrophilicity/hydrophobicity，protein structure，phosphorylation sites and transmembrane region of chicken PLA2G7 at the molecular level，and combined with protein interaction network analysis for comprehensive evaluation. The results showed that the chicken PLA2G7 gene was 1 923 bp in length and encoded a protein consisting of 641 amino acids，which was mainly distributed in the cytoplasm and belonged to a hydrophilic secreted protein； The secondary structure is mainly composed of irregular coil（48.36%），α ⁃ helix（31.36%），extended chain （12.79%）and β⁃Sheet（7.49%）. Functional prediction revealed that the protein has 46 potential phosphorylation sites，29 of which may be serine phosphorylation sites. Based on the STRING protein interaction network，four proteins involved in immune response and regulation of phospholipid metabolism were preliminarily analyzed. Conclusion：the sequence characteristics and structure of the protein encoded by PLA2G7 gene reveal that it may be closely related to the regulation of phospholipid metabolism and immune response in chickens. This finding provides a theoretical basis for further exploring the functional mechanism of Lp⁃PLA2.

Key words: Chicken, PLA2G7, Bioinformatics, Lp?PLA2

摘要： PLA2G7 基因编码的脂蛋白相关磷脂酶A2(Lp?PLA2)主要由炎症细胞分泌，与许多代 谢、免疫功能障碍等疾病有关。该研究采用生物信息学方法从分子水平对鸡PLA2G7蛋白的理 化性质、亲水性/疏水性、蛋白质结构、磷酸化位点及跨膜区等多方面进行结构分析，结合蛋白 互作网络分析进行综合评价。结果表明：鸡PLA2G7 基因全长1 923 bp，编码的蛋白质由641个 氨基酸组成，主要分布于细胞质，属于亲水分泌型蛋白；二级结构以无规则卷曲（48.36%）为 主，还有α?螺旋（31.36%）、延伸链（12.79%）和β?折叠（7.49%）形式组成。功能预测发现，该蛋 白有46个潜在的磷酸化位点，其中29个可能是丝氨酸磷酸化位点。通过STRING蛋白互作网 络推测，其与4个免疫反应和磷脂代谢相关蛋白存在互作关系。结论：PLA2G7 基因编码蛋白的 序列特征和结构揭示其可能与鸡的磷脂代谢调控以及免疫应答过程有密切关系，为进一步探 讨Lp?PLA2的功能机理提供了理论依据。

关键词: 鸡, PLA2G7基因, 生物信息学, Lp-PLA2