Abstract: Pseudorabies，also called Aujeszky 's disease，is a highly contagious disease caused by pseudorabies virus (PRV). In the absence of a specific host，pseudorabies virus can invade the body of a variety of mammals， including pigs，cattle，sheep，etc.，which leads to severe clinical symptoms and acute death in susceptible animals. The existence of porcine pseudorabies was first reported in China in the 1950s，and since 2011，new porcine pseudorabies variants have emerged in some areas，resulting in serious economic losses to the pig industry. Although scientists have been working on the design of diagnostic methods and the development of related vaccines， pseudorabies is still widespread in the pig industry，and its discovery in recent years that it also has potential problems for human health has aroused strong concern in the world. At present，pseudorabies vaccine is widely immunized in pig farms in China，so it is very important to detect the antibody level produced by the vaccine after immunization. In this paper，the current antibody detection methods for PRV will be summarized，and their advantages anddisadvantages will be compared and analyzed in order to provide a reference for the selection of antibody detection methods on pig farms.

Key words: Pseudorabies virus；Antibody testing；VNT；ELISA

摘要： 伪狂犬病，也称阿氏病（Aujeszky's disease），是由伪狂犬病毒（PRV）引起的一种高 度传染性疾病。在没有特定宿主的情况下，伪狂犬病毒可以侵入多种哺乳动物，包括猪、牛、 羊等，从而导致易感动物发生严重的临床症状和急性死亡。我国于 20 世纪 50 年代首次报道猪 伪狂犬病，且自 2011 年以来，部分地区出现新的猪伪狂犬病变异株，致使养猪业遭受严重的 经济损失。尽管科学家们一直在致力于诊断方法的设计和相关疫苗的开发，但伪狂犬病仍在 养猪业广泛存在，近些年发现其对人类的健康也存在着潜在威胁，从而引起了世界范围内的 强烈关注。目前我国的猪场普遍实行伪狂犬疫苗免疫，因此检测免疫后疫苗产生的抗体水平 至关重要。本文总结目前对 PRV 的抗体检测方法，针对其优缺点进行对比分析，以对猪场临 床上选择抗体检测方法提供参考。

关键词: 伪狂犬病毒；抗体检测；病毒中和试验；ELISA