Abstract: To investigate the impact of cryopreservation conditions on the detection of antibodies against Salmonella pullorum and the feasibility of using residual plasma after avian leukosis detection for pullorum disease detection，this study selected 3 860 three⁃yellow chickens for pullorum disease testing in batches. The detection results using different anticoagulants，cryopreserved blood samples，serum，and residual plasma were compared with the standard on⁃site whole blood plate agglutination detection. The results showed that the positive detection rates of lithium heparin anticoagulated blood，ethylenediaminetetraacetic acid dipotassium salt（EDTA dipotassium salt， EDTA ⁃ 2K）anticoagulated blood and residual plasma were highly consistent with the on ⁃ site whole blood plate agglutination detection results. The positive individuals detected by the on⁃site whole blood plate agglutination method could be completely detected in both cryopreserved and non⁃cryopreserved serum and residual plasma samples. The positive detection rate of serum samples was slightly higher than the standard method，and short⁃term cryopreservation had no significant adverse effect on the detection results. In addition，the actual application effect of using residual plasma for pullorum purification was evident.

Key words: Chicken dysentery detection, Plasma residual fluid, Frozen conditions

摘要： 为探究冻存条件对鸡白痢沙门氏菌抗体检测的影响以及利用用于检测禽白血病的 血浆分离后剩余残液用于鸡白痢抗体检测的可行性，该研究选用3 860只三黄鸡分批次进行鸡 白痢抗体检测试验，比较使用不同抗凝剂、冻存血样、血清、血浆残液所检测结果与标准现场全 血平板凝聚检测的差异，结果表明：肝素锂抗凝血、乙二胺四乙酸二钾（EDTA dipotassium salt； EDTA?2k）抗凝血、血浆残液的阳性检出率与现场全血平板凝聚检测结果高度吻合，现场全血 平板凝集法检出的阳性个体在其常温/冻存血清、常温/冻存血浆残液样品中均能完全检出，血 清样品的阳性检出率较标准法偏高，并且短期冻存对于检测结果并无明显不利影响；此外，利 用血浆残液进行鸡白痢净化所表现出来的实际应用效果明显。

关键词: 鸡白痢抗体, 检测, 血浆残液, 冻存条件