Abstract: Bovine tuberculosis is a chronic zoonotic infectious disease caused by Mycobacterium bovis tuberculosis，which can occur all the year round and is classified as a classⅡ animal disease in China. In 2020，A case of bovine tuberculosis occurred in a dairy farm in Guiping City，16 positive samples were detected by intradermic allergy test. And then，the anticoagulant blood samples of 16 positive cows were collected and tested with ELISA of bovine tuberculosis interferon gamma（IFN），the results showed 8 positive cows. Infected cows were culled by Guiping center for animal disease prevention and control. Therefore，it is suggested that quarantine and biosafety measures should be taken to prevent the occurrence of bovine tuberculosis. In the monitoring link，intradermal allergy can be used to quarantine cattles，and then retesting positive cattles using IFN??γELISA method. It will be more conducive to the detection and purification of bovine tuberculosis.

Key words: Bovine tuberculosis； Diagnosis； Management

摘要： 牛结核病是一种由牛型结核分枝杆菌引起的慢性人兽共患传染病，该病一年四季 均可发生，被我国列为二类动物疫病。2020年，桂平市某奶牛场发生疑似牛结核病，经皮内变 态反应检测出阳性样品16份。采集这16份皮内变态反应阳性牛的抗凝血样采用ELISA方法进 行牛结核病γ??干扰素检测，结果诊断出8头牛结核病阳性奶牛。感染奶牛由桂平市动物疫病 预防控制中心进行扑杀处理。据此建议，养殖场应该做好检疫和生物安全措施，防止该病的 发生，在监测环节中可先用皮内变态反应检疫牛群，对于阳性牛再结合γ?干扰素ELISA方法进 行综合判断，将更有利于牛结核病的检测和净化。

关键词: 牛结核病； 诊断； 处理