Abstract: Eighty 6?month?old poodle puppies were randomly divided into 8 groups（groups A to H）according to the principle of similar body weight，with 10 dogs in each group. The poodle puppies in group A were fed with based diet，the poodle puppies in group B to H were fed with based diet plus inactivated Lactobacillus acidophilus，Bacillus coagulans，plant Lactobacillus，mixture of inactivated Lactobacillus acidophilus and Bacillus coagulans，mixture of inactivated Lactobacillus acidophilus and plant Lactobacillus，mixture of Bacillus coagulans and plant Lactobacillus， mixture of inactivated Lactobacillus acidophilus and Bacillus coagulans and plant Lactobacillus，respectively. The pre? feeding period was 7 days and the test period was 30 days. The results were as follows：1）With no difference in daily feed intake，the average daily gain of puppies was different among groups（P < 0.05），and the dogs in group H was significantly higher than those in the control group and the other six experimental groups（P < 0.05），except for group D（P > 0.05）；2）There was a highly significant difference in fecal score among all groups（P < 0.01）. The lowest fecal score and no dilute defecation were found in group B，group H and group G；3）The fecal lactobacillus content in groups C，E and H continued to increase，and group H had the largest increase and the highest content；4）The apparent digestibility of crude protein in group D was significantly higher than that in groups C，F and G（P < 0.05）， and highly significantly higher than that in groups A and H（P < 0.01）. In conclusion，the combination of threeprobiotics can significantly promote the growth and development of poodle puppies，improve intestinal health and promote the proliferation of intestinal lactobacillus.

Key words: Probiotics； Puppy； Average Daily Gain； Dung Scores； Apparent Digestibility； Lactobacillus

摘要： 试验将80只6月龄的贵宾幼犬按体重相近原则随机分为8组（A~H组），每组10只 犬。A组饲喂基础日粮，B~H在饲喂基础日粮的基础上，分别添加灭活嗜酸乳杆菌、凝结芽孢 杆菌、植物乳杆菌、灭活嗜酸乳杆菌+凝结芽孢杆菌、灭活嗜酸乳杆菌+植物乳杆菌、凝结芽孢杆 菌+植物乳杆菌、灭活嗜酸乳杆菌+凝结芽孢杆菌+植物乳杆菌。预饲7天，试验周期为30天。 试验结果表明：1）在日采食量无差异的情况下，不同组别的幼犬平均日增重存在组间差异性 （P<0.05），其中 H 组显著高于对照组和其余 6 个试验组（P<0.05），与 D 组无显著差异（P＞ 0.05）；2）各组间粪便评分有极显著差异（P<0.01），粪便评分最低与软便率为0的三组依次为B、 H、G组；3）C、E、H组的粪便乳酸杆菌含量持续增长，H组增值幅度最大，含量最高；4）D组粗蛋 白的表观消化率显著高于C、F、G组（P<0.05），极显著高于A和H组（P<0.01）。结论：三菌联用 效果具有显著的促进幼犬生长发育、改善肠道健康与促进肠道乳酸杆菌增殖的作用。

关键词: 益生菌； 幼犬； 平均日增重； 粪便评分； 表观消化率； 乳酸杆菌