Abstract: In order to study the protective effect of polysaccharide of Atractylodes macrocephala Koidz（PAMK） on the liver immune function of goslings，40 1? day ?old Magang goslings were randomly divided into control group， PAMK group，CTX group and PAMK+CTX group，10 in each group. After 3 days of feeding，400 mg/kg PAMK was added to the diet of control group and PAMK group. At 20 days of age，CTX group and PAMK + CTX group were injected with cyclophosphamide in leg muscle at a dose of 40 mg/kg·BW for three consecutive days，other groups were injected with 0.5 mL of normal saline. The liver index and tissue structure were detected on 24?day ?old. The resultsshowed that cyclophosphamide could cause liver enlargement，tissue structure damage，hepatocyte DNA damage and liver function disorder in goslings. After the intervention of PAMK，the gosling liver and gallbladder indexes returned to normal，and the serum AST and AST levels were significantly reduced. The activity of ALT（P<0.05），the contents of TNF ? α，IL ? 10 and IL ? 1β were significantly increased（P<0.05），and the DNA damage of hepatocytes was significantly reduced. Cyclophosphamide significantly inhibited the protein levels of TLR4，NF ? κB1，TRAF6 and IKKε in the TLR4/NF ? κB signaling pathway（P<0.05），and increased the protein level of IκBα（P<0.05）. After PAMK treatment，the protein level of IκBα was significantly decreased（P<0.05），and the protein level of TRAF6 was significantly increased （P<0.05）， indicating that PAMK could effectively relieve the inhibitory effect of cyclophosphamide on key genes of TLR4/NF?κB pathway.

Key words: Polysaccharide of Atractylodes macrocephala Koidz（PAMK）, gosling, liver, cyclophosphamide, immune function, TLR4

摘要： 为研究白术多糖对雏鹅肝脏免疫功能的保护作用，将40只1日龄马岗鹅幼雏随机 分为control组、PAMK组、CTX组和PAMK+CTX组，每组10只，预饲3 d后，control组和CTX组 饲喂基础饲粮，PAMK组和PAMK+CTX组在基础饲粮中添加400 mg/kg白术多糖；20日龄时， CTX组和PAMK+CTX组以40 mg/kg·BW剂量连续三天腿肌注射环磷酰胺，其余组注射0.5 mL 生理盐水，注射期3天，第24日龄采样。结果显示：环磷酰胺可导致雏鹅肝脏肿大，组织结构 损伤，肝细胞DNA损伤及肝功能紊乱；经白术多糖干预后，雏鹅肝脏和胆囊指数恢复至正常， 显著降低了血清中AST和ALT的活性（P<0.05），TNF?α、IL?10和IL?1β含量显著升高（P<0.05）， 肝细胞DNA损伤明显减轻。环磷酰胺对TLR4/NF?κB信号通路中的TLR4、NF?κB1、TRAF6和 IKKε蛋白水平有明显的抑制作用（P<0.05），并使IκBα蛋白水平升高（P<0.05），经白术多糖处 理后，IκBα蛋白水平显著下降（P<0.05），TRAF6蛋白水平显著升高（P<0.05），表明白术多糖可 有效解除环磷酰胺对TLR4/NF?κB通路关键基因的抑制作用。

关键词: 白术多糖, 鹅, 肝脏, 环磷酰胺, 免疫功能, TLR4