Eighty 6?month?old poodle puppies were randomly divided into 8 groups（groups A to H）according to the principle of similar body weight，with 10 dogs in each group. The poodle puppies in group A were fed with based diet，the poodle puppies in group B to H were fed with based diet plus inactivated Lactobacillus acidophilus，Bacillus coagulans，plant Lactobacillus，mixture of inactivated Lactobacillus acidophilus and Bacillus coagulans，mixture of inactivated Lactobacillus acidophilus and plant Lactobacillus，mixture of Bacillus coagulans and plant Lactobacillus， mixture of inactivated Lactobacillus acidophilus and Bacillus coagulans and plant Lactobacillus，respectively. The pre? feeding period was 7 days and the test period was 30 days. The results were as follows：1）With no difference in daily feed intake，the average daily gain of puppies was different among groups（P < 0.05），and the dogs in group H was significantly higher than those in the control group and the other six experimental groups（P < 0.05），except for group D（P > 0.05）；2）There was a highly significant difference in fecal score among all groups（P < 0.01）. The lowest fecal score and no dilute defecation were found in group B，group H and group G；3）The fecal lactobacillus content in groups C，E and H continued to increase，and group H had the largest increase and the highest content；4）The apparent digestibility of crude protein in group D was significantly higher than that in groups C，F and G（P < 0.05）， and highly significantly higher than that in groups A and H（P < 0.01）. In conclusion，the combination of threeprobiotics can significantly promote the growth and development of poodle puppies，improve intestinal health and promote the proliferation of intestinal lactobacillus.