Abstract: The detection of serum antibodies in the veterinary laboratory mainly adopts the ELISA detection method. This paper discusses the common problems in the daily ELISA test based on the actual operation summary of the veterinary laboratory. It is found that the influencing factors of the common problems in the ELISA test include sample processing，experimental operation methods，experimental equipment，and so on. It is suggested that the ELISA operation method must be strictly mastered in veterinary laboratory testing to reduce or avoid experimental failures caused by operational errors. This article can provide help for veterinary laboratory colleagues.

Key words: ELISA detection； Veterinary laboratory； Problem； Discussion

摘要： 兽医实验室中血清抗体的检测主要采用ELISA检测方法，本文结合兽医实验室的实 际操作总结对日常ELISA检测中常出现的问题进行探讨，发现ELISA检测中常见问题的影响 因素包括样品的处理、实验操作方法、实验仪器设备等。提示在兽医实验室检测中必须严格掌 握ELISA操作方法，减少或避免因操作失误而导致的实验失败，本文可为兽医实验室同行提供 帮助。

关键词: ELISA检测； 兽医实验室； 问题； 探讨