Guangdong Journal of Animal and Veterinary Science ›› 2023, Vol. 48 ›› Issue (1): 63-70.DOI: 10.19978/j.cnki.xmsy.2023.01.12

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Molecular epidemiological investigation of transmissible viral proventriculitis in poultry farms located in western Guangdong

PAN Xiaoting1，2# ，LI Xiu2，3# ，QI Nanshan2 ，LIAO Shenquan2 ，LI Juan2 ，LV Minna2 ，LIN Xuhui2 ，CAI Haiming2 ， HU Junjing2 ，ZHONG Yaoguang4 ，ZHUO Fuqiu4 ，ZHANG Jianfei2，SUN Mingfei2* ，LI Guoqing3*   

  1. （1.Foshan University，Foshan Guangdong 528225； 2.Maoming Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology，KeyLaboratory of Livestock Disease Prevention of Guangdong Province，Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province，Key Laboratory of Avian Influenza and Other Major Poultry Diseases Prevention and Control，Ministry of Agriculture and Rural Affairs，Institute of Animal Health， Guangdong Academy of Agricultural Sciences，Guangzhou Guangdong 510640； 3. South China Agricultural University，Guangzhou Guangdong 510642； 4.Guangdong new pilot ecological agriculture development Co.，Ltd，Guangdong Zhaoqing 526300）
  • Online:2023-02-21 Published:2023-02-18

粤西地区规模化养鸡场鸡传染性病毒性腺胃炎分子流行病学调查研究

潘晓婷1，2#，李秀2，3#，戚南山2，廖申权2，李娟2，吕敏娜2，林栩慧2，蔡海明2，胡俊菁2，钟耀光4，卓福球4，张健騑2，孙铭飞2*，李国清3*   

  1. （1.佛山科学技术学院，广东 佛山 528000； 2.广东省农业科学院动物卫生研究所/岭南现代农业科学与技术广东省实验室茂名分中心/农业 农村部兽用药物与诊断技术广东科学观测实验站/广东省畜禽疫病防治研究重点实验室， 广东 广州 510640； 3. 华南农业大学，广东 广州 510642； 4.广东新领航生态农业发展有限公司，广东 肇庆 526300）
  • 通讯作者: 孙铭飞，李国清
  • 基金资助:
    广东省自然科学基金（2021A1515010521，2021A1515012401，2020A1515011575，2019A1515010913）；广东省科技计划项目 （2021B1212050021）；河源市科技计划项目（2021008）；云浮市科技计划项目（2021040202，2021020604，2021020607）；广东省现代农业产业 技术体系创新团队建设专项（2019KJ119）；科技创新战略专项资金（高水平农科院建设）（202110TD，202122TD，R2021PY?QY007）

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Abstract: In order to investigate the prevalence and main causes of transmissible viral proventriculitis（TVP）in chickens in western Guangdong，we collected a total of 910 glandular gastric samples（every 10 mixed into 1 pool sample，a total of 91 pool samples）from some chicken farms in western Guangdong province，and detected 8 kinds of TVP ? related viruses using real ?time PCR method. The results indicated that 42 out of the 91 samples were positive， with a positive rate of 46.2%，and a total of 6 kinds of TVP ? related viruses were detected，and double or multiple infection were found. There is no breed specificity for infected chickens in this area，but infection rate among different age groups varies significantly. It suggests that TVP infection is widespread in chicken farms in western Guangdong province，and our data will provide basic information for the epidemic characteristics as well as prevention and control of TVP in Western Guangdong.

Key words: qPCR, Infectious viral adenatitis, Western Guangdong

摘要： 为了解广东粤西地区鸡传染性病毒性腺胃炎（Transmissible viral proventriculitis， TVP）的流行情况和主要病因，本研究于粤西地区部分发病鸡场共采集910份（每10份混为1个 样本，共91个样本）腺胃样品，使用实时荧光定量PCR检测方法对8种TVP相关病毒进行检 测。结果显示，91份样品中检测出42份相关病毒核酸阳性，阳性率46.2%；共检出TVP相关病 毒6种，发现有病毒双重感染和混合感染情况。该地区病鸡无品种特异性，但小鸡和中鸡病毒 感染率差异显著。这表明粤西地区养鸡场普遍存在TVP感染，本研究为粤西地区TVP的流行 规律研究及防控提供基础数据。

关键词: 荧光定量PCR, 传染性病毒性腺胃炎, 粤西地区

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