关键词: 公鸡；睾丸；白消安；生殖细胞

Abstract: To develop a method suitable for preparing chicken reproductive precursor cell transplant recipient rooster. In this experiment，81 Lingnan yellow roosters with similar body weight and normal reproductive performance were selected for the experiment. The test was carried out in two times. The first test was to calculate the busulfan dosage according to the body weight of the roosters. Direct injection of busulfan into males was evaluated by analyzing blood routine indexes，body weight，testicular weight，mortality，diameter of seminiferous tubules，circumference andarea，and fertility of males on day 15，30，45 or 60 after injection of busulfan. Effects of a method for preparing chicken reproductive precursor cell transplant recipients in the testes of young roosters. The results of test 1 showed that only one rooster with an injection dose of 1.2 mg/kg showed decreased spermatogenic cells in the seminiferous tubules of the testis on the 15th day after the injection of busulfan. Size and weight and fertilization rates were not affected，while other birds showed no significant change compared to the control group. The results of test 2 showed that the size and weight of the testis were significantly reduced on the 15th and 30th days after injection of 30 mg/ unilateral to 50 mg/unilateral busulfan into the testis of roosters. The tissue section analysis after the 30th day showed that the seminiferous tubule membrane in the testis was obtained. The bottom and gland cavity were emptied，and most spermatogonial stem cells in the testicles of males injected with an injection dose of 30 mg/unilateral to 40 mg/ unilateral on the 45th day could be recovered. Fertilization rate decreased significantly，but rooster body weight and blood routine indexes had no significant effect during the whole process. In conclusion，the testicular injection of busulfan can clear endogenous spermatogonial stem cells，and the normal physiological reproductive function of the testis is not affected. The injection dose is 30 mg/unilateral to 40 mg/unilateral on the 30th day after ，the bottom of seminiferous tubule membrane and gland cavity in the testis of rooster are emptied，and this time is the best time to inject reproductive progenitor cells.