关键词: 新型鹅星状病毒, qPCR, ORF2

Abstract: In order to develop a credible dual fluorescence quantitative polymerase chain reaction（qPCR）for detecting novel goose astrovirus type 1（NGAstV⁃1）and type 2（NGAstV⁃2），primers were designed to establish a dual fluorescence qPCR detection method using ORF2 protein of NGAstV⁃1 and NGAstV⁃2，and 36 samples of goose liver and kidney tissue suspected to be infected with NGAstV⁃1 and NGAstV⁃2 were evaluated in this study. The test results showed that the correlation coefficients of qPCR were 0.997 and were negative for novel duck reovirus （NDRV），avian influenza virus（AIV），fowl adenovirus（FAdV），and goose parvovirus（GPV）. The minimum detection limit was 1.12 × 101 copies/μL. The reproducibility test results indicate good reproducibility，with a coefficient of variation between groups of less than 0.8% and a coefficient of variation within groups of less than 0.5%. It can be seen that the qPCR can efficiently and specifically detect NGAstV⁃1 and NGAstV⁃2，providing a feasible method for NGAstV monitoring.

Key words: Novel goose astrovirus, qPCR, ORF2