This test was researched for the pathogenic bacteria and drug resistance of dead pigs in a pig farm in Anning，Yunnan Province. Collecting liver，lung，spleen，and lymph nodes of dead pigs for smear staining， microscopic examination，separation culture，biochemical test，animal pathogenicity test，16S rRNA identification， serotype identification，virulence factor genotype and phenotype detection，and drug sensitivity test to study the isolated strains. The results showed that the Ynanning SS from the organs of dead pigs was Streptococcus suis type 2. The 16S rRNA gene sequence of Yinanning SS was 99.3% homologous to LC377187 and KM972351，and was higher than 92.5% homologous to other Streptococcus suis. Ynanning SS is resistance to gentamicin，clindamycin， lincomycin，cefazolin，cefuroxime，ceftazidime and sulfamethoxam，moderately sensitive to penicillin，ciprofloxacin， and sensitive to doxycycline，ceftriaxone，cefotaxime，tetracycline and sulfadiazine. Selecting sensitive drugs toclinical isolates can achieve good therapeutic effects. The research results provide a certain reference for the prevention and treatment of local Streptococcus suis disease.