Canine parvovirus（CPV）positive serum is indispensable in the efficacy test，exogenous virus testThe results showed that the neutralizing antibody titer of CPV positive serum was as high as 1∶32768，the neutralizing index was higher than 106.2 ，the neutralizing antibody titers of CPV positive serum against canine distemper virus， canine parainfluenza virus and canine adenovirus were all lower than 1∶4，and the neutralizing antibody titer of rabies virus was lower than 0.03 IU/mL. The CPV positive serum can completely neutralize 10 doses of vaccine virus in exogenous virus test for three CPV related live vaccines. The CPV positive serum was used for identification test of two CPV related live vaccines，and the results met the quality standards. IFA for CPV was used to determine the viral titer of three CPV vaccine strains，and the results showed that the IFA method established had cross immune response to the three vaccine strains，which had little difference with the sensitivity of commercial CPV fluorescent monoclonal antibody. All above show that CPV positive serum has strong neutralizing ability and good specificity，and can be used for the efficacy test，exogenous virus test and identification test of canine live vaccines. and identification test of CPV ? related vaccines，and is crucial for the quality control of CPV ? related biological products. In order to prepare CPV positive serum with high neutralizing antibody titer and good specificity，two healthy and susceptible beagles were immunized with CPV DD strain for the test. After four times of immunization，we collected blood and separated serum. The serum was identified by virus neutralization test，immunofluorescence assay，and sterility test，mycoplasma test，and exogenous virus test in the third part of the Chinese Veterinary Pharmacopoeia 2020. And then the serum was used in the exogenous virus test and identification test in canine vaccines. An indirect immunofluorescence assay（IFA）for CPV was established and applied to potency test of vaccine.