Abstract: Temperature is an important factor affecting the sporulation and viability of avian coccidia oocysts. In this study，the effects of different temperatures on the sporulation of Eimeria tenella unsporulated oocysts and the viability of sporulated oocysts were investigated by monitoring the sporulation rate of avian coccidia oocysts，intestinal lesions and oocyst production. The results showed that there was no significant change in the oocyst sporulation activity compared to be treated at -80 ℃ for 30 min，and at -40 ℃，-20 ℃，0 ℃，4 ℃，10 ℃，28 ℃，40 ℃，50 ℃ for 30 min or 1 h respectively，but the oocyst sporulation activity was inhibited after administrated at -80 ℃ for more than 1 h，- 40 ℃ or - 20 ℃ for more than 48 h，and 60 ℃ for more than 30 min. The sporulated oocysts were killed by exposure to liquid nitrogen for 2 min，or at -80 ℃ or higher than 50 ℃ for more than 1 h，whereas sporulated oocysts remain viable at -20 ℃ for 1 to 12 h or at 50 ℃ for 30 min. This study provides data support for the storage conditions of avian coccidia oocysts，and has certain research significance for the prevention and treatment of avian coccidiosis.

Key words: Eimeria tenella, Temperature, Vitality, Sporulation

摘要： 环境温度是影响鸡球虫卵囊孢子化和活力的重要因素，该研究通过监测鸡球虫卵 囊孢子化率、鸡感染球虫后的肠道病变以及卵囊排出情况，探讨不同温度对柔嫩艾美耳球虫未 孢子化卵囊的孢子化作用以及对孢子化卵囊的活性影响。结果表明：未孢子化卵囊分别经过 -80 ℃处理 30 min，-40 ℃、-20 ℃、0 ℃、4 ℃、10 ℃、28 ℃、40 ℃、50 ℃处理 30 min 或 1 h 后，孢子化活性均无显著变化；未孢子化卵囊分别经过-80 ℃处理1 h以上、-40 ℃或-20 ℃处 理48 h以上、60 ℃处理30 min以上后孢子化活性被抑制；孢子化卵囊经液氮处理2 min、-80 ℃ 或高于50 ℃条件处理超过1 h，卵囊活力基本为0，在-20 ℃处理1—12 h或50 ℃处理30 min 卵囊仍表现有一定活力。该研究为鸡球虫卵囊保存条件提供数据支持，对防治鸡球虫病具有 一定的研究意义。

关键词: 柔嫩艾美耳球虫, 不同温度, 卵囊活力