Abstract: In order to establish a rapid and accurate detection method for African swine fever（ASF），a standarddetection of ASFV，it was found that the primers and probes provided by CADC were more sensitive to the detection of ASFV，and the DNA extraction kit of Axygen brand was more cost?effective for the detection of ASFV. plasmid and a standard curve were constructed for the late translation protein p72 gene of ASFV. The sensitivity of primers and probes provided by OIE and CADC was compared by fluorescence quantitative PCR technology，and the detection efficiency of ASFV DNA extracted by various DNA extraction kits was evaluated，providing data reference for the efficient detection of ASFV. The results showed that p72 standard plasmid had better detection efficiency and sensitivity among the standard plasmid and standard curve of p72 gene of ASFV constructed. Through the use of primers， probes and various DNA extraction kits provided by OIE and CADC for fluorescence quantitative PCR

Key words: African swine fever virus, p72, Standard curve, DNA extraction

摘要： 为建立快速、准确的非洲猪瘟（African swine fever，ASF）检测方法，试验针对ASFV 的晚期翻译p72蛋白基因构建了标准品质粒和标准曲线，并利用荧光定量PCR技术对OIE和 CADC提供的引物和探针进行灵敏性的比对及对多种DNA提取试剂盒提取的ASFV DNA检测 效率进行了评价，为ASFV的高效检测提供数据参考。结果表明：构建的ASFV晚期p72基因的 标准品质粒和标准曲线中，p72标准品质粒的检测效率与灵敏度较优。通过使用OIE、CADC分 别提供的引物、探针和多种DNA提取试剂盒进行ASFV荧光定量PCR检测，发现CADC提供的 引物和探针对于ASFV的检测更加灵敏，Axygen品牌的DNA提取试剂盒对ASFV的检测性价比 更高。

关键词: 非洲猪瘟病毒, p72, 标准曲线, DNA提取试剂盒