关键词: 地方猪, 体细胞核移植, 克隆, 遗传资源保护

Abstract: This study aimed to clone the Lantang pig using somatic cell nuclear transfer technology，offering a novel and efficient approach for preserving the genetic resources of exceptional local and endangered pig breeds in China. We compared the tissue block method and collagenase digestion method to establish Lantang pig somatic cell lines. Additionally，we analyzed the developmental outcomes of cloned embryos created from Lantang pig donor cells of different genders in vitro and transplanted them in vivo. The results indicated that the collagenase method yielded cells more rapidly and in higher quantities compared to the tissue block method. While there were no notable differences in cleavage rates and total blastocyst cell counts between cloned embryos of different genders in vitro，the blastocyst rate of male embryos was significantly higher than that of female embryos（P<0.05）. Through in vivo transfer of cloned embryos from Lantang pigs in three separate batches，we successfully obtained four healthy cloned piglets. This study marks the first successful case of somatic cell cloning of the Lantang pig in China，providing a dependable technical tool for preserving the genetic resources of this breed.

Key words: local pig breeds, Somatic Cell Nuclear Transfer, cloning, Protection of Genetic Resources