关键词: 宏病毒组学；流浪犬；DNA病毒群落；功能基因分析

Abstract: To investigate the composition and functional potential of DNA viral communities in stray dog blood， this study employed viral metaviromics to perform high?throughput sequencing of blood samples collected from stray dogs at a rescue center in Shenzhen. Host?derived sequences were filtered and removed，followed by analysis of short? sequence reads and assembled contigs to characterize the DNA viral community structure. The results revealed that the dominant viral orders were Herpesvirales，Caudovirales，and Cirlivirales，while the predominant genera were Varicellovirus and Circovirus. Functional gene annotation was conducted using the KEGG and COG databases. KEGG analysis identified seven major functional categories，and COG classification covered 20 functional groups. These functional categories primarily included mobile genetic elements，general function prediction，genetic information processing，multi?level functional structures，metabolism，and transcription. This study demonstrating that the DNA virome in stray dog blood from the Shenzhen region is dominated by herpesviruses，circoviruses，and bacteriophages， suggesting potential widespread immunosuppression and co?infection risk within the local stray dog population. These findings highlight significant indirect public health implications and provide a foundation for further exploration of the pathogenicity and functional roles of DNA viruses in stray dog blood.

Key words: Metaviromics；Stray dogs；DNA viral communities；Functional annotation of genes