关键词: 化学风险物质, 筛查与确证, 四级杆/静电场轨道阱高分辨质谱

Abstract: This paper describes the development of mass spectrometry library and the method of screening and confirming 83 drugs and their metabolites in black⁃bone chicken meat，eggs，beef，and mutton using ultra⁃high performance liquid chromatography ⁃ quadrupole ⁃ obitrap high resolution mass spectrometry （UHPLC ⁃ Q/Obitrap HRMS）. A two⁃step procedure was employed：homogenized samples were first extracted with 2 mL aqueous solution， followed by a second extraction using 8 mL of acetonitrile. The extract was then passed through a purification column， reconstituted after nitrogen blow⁃down，and analyzed by UHPLC⁃Q/Obitrap. The screening detection limits ranged from 5.0－ 20 μg/kg. Average recoveries across three spiked concentration levels were 60%－ 110%. This method proved to be straightforward，rapid and reliable，making it suitable for the rapid screening and confirmation analysis of veterinary drug residues in black⁃bone chicken meat，eggs，beef and mutton.

Key words: Chemical risk substances, Screening and confirming, UHPLC?Q/Obitrap HRMS