关键词: 类器官是指由细胞组成的类似器官的结构。肠道类器官是一个由肠道干细胞经过 体外培养得到的多种细胞组成的三维结构。近年来, 肠道类器官在医学、药物筛选和动物科学 的研究领域受到了关注。由于能较好还原体内生理状况, 肠道类器官是替代细胞作为体外研 究的有力工具。 在猪基础研究上能模拟病毒感染、肠道生理状况等, 现已用于营养吸收和病毒疾病等的研 究。近年来, 畜牧兽医领域的一些团队逐渐采用肠道类器官进行研究, 近年来相关文章数也 在逐年上升, 可见其在猪基础研究上逐渐展露出其优势, 其应用前景可观, 但进一步扩增其应 用还有一些悬而未决的问题, 因此该文综述了肠道类器官在动物科学特别是猪研究上的应 用, 并提出其在畜牧兽医领域一些发展建议。

Abstract: Intestinal organoids are cell ? based structures that mimic the functions of organs. They are three ? dimensional structures composed of various types of cells derived from intestinal stem cells via laboratory cultivation. In recent years，their applications in medical research，drug testing，and animal sciences have drawn significant attention. Intestinal organoids offer a promising tool for studying the physiology of the gut as they closely resemble the in vivo conditions and as reliable alternatives to traditional cell?based models in vitro research. In swine?based studies， these organoids have been successfully employed to simulate viral infections and investigate intestinal functions， including nutrient absorption and viral diseases.The use of intestinal organoids has also gained attention in the field of veterinary medicine. Their potential and advantages have been increasingly recognized，leading to a rise in related publications. However，there are still unresolved challenges that need to be addressed to further expand their applications in this field.In this review article，we provided an overview of the applications for intestinal organoids in animal sciences，with a specific focus on swine research. Furthermore，we proposed several recommendations for the development and future directions of this technology in the field of livestock and veterinary medicine.

Key words: Intestinal organoids, Cell culture, Swine