关键词: 亚硒酸钠, 硒蛋白, 细胞增殖, SelW, SBP1

Abstract: This experiment was designed to investigate the effects of different concentrations of sodium selenite on the proliferation and differentiation of porcine skeletal muscle satellite cells and on the expression of SelW and SBP1. The skeletal muscle satellite cells were isolated from white pigs，and the differentiation of skeletal muscle satellite cells and their status were identified by immunohistochemistry. The skeletal muscle satellite cells were cultured at five concentrations of sodium selenite：0（control group），0.02（0.02% group），0.1（0.1% group），0.5 （0.5% group），and 2.5（2.5% group）μg/mL，and the cell proliferation and differentiation were observed on days 1 to 4，respectively. Cells which were cultured by the abovementioned different concentration of sodium selenite were collected on day 1，3，5，and 7 respectively and RNA were extracted for detecting the expression of SelW and SBP1 gene. The results showed that：1）From day 1 to day 4，cells proliferation in the 0.02%，0.1%，and 0.5% groups were similar to that of the control group，with the 0.1 ug/mL group showing the closest trend to the control group. However， the number of cells in the 2.5% group decreased after day 3 and massive cells death occurred. 2）The expression of SelW was mostly affected by different concentrations of sodium selenite on day 3，showing a trend of first upregulation and then downregulation，along with increased concentration of sodium selenite. Data of day 3 showed that the mRNA expression of SelW was significantly increased in the 0.02% ，0.1% ，0.5% and 2.5mL groups compared with the control group，and the expression in the 0.1% and 0.5% groups was higher than that in the 0.02% and 2.5% groups （P<0.05）. The expression of SBP1 was mostly affected by different concentrations of sodium selenite on day 3 and day 7，showing a trend of increasing and then decreasing with increasing sodium selenite concentration. At day 3， compared with the control group，the mRNA expression of SBP1 was significantly increased in the 0.02% and 0.5% groups，the expression in the 0.02% group was significantly higher than that in the 0.1% and 2.5% groups，and the expression in the 0.5% group was significantly higher than that in the 0.1% group（P<0.05）. At day 7，the mRNA expression of SBP1 in the 0.1% and 0.5% groups was significantly higher than that in the control，0.02% and 2.5% groups，and the expression in the 2.5% group was lower than that in the control group（P<0.05）. In conclusion，low concentrations of sodium selenite at 0.02% and 0.1% did not inhibit and impair cell proliferation，while 0.5% sodium selenite level inhibited cell proliferation rate and 2.5% sodium selenite level had strong cytotoxicity. The mRNA expression of SelW and SBP1 was promoted by low concentrations of sodium selenite at 0.02% and 0.1%，whereas the mRNA expression of SelW and SBP1 was inhibited by 2.5% concentration of sodium selenite.

Key words: Sodium selenite； Selenoprotein, cell； Proliferation； SelW； SBP1