关键词: 白术多糖, 鹅, 肝脏, 环磷酰胺, 免疫功能, TLR4

Abstract: In order to study the protective effect of polysaccharide of Atractylodes macrocephala Koidz（PAMK） on the liver immune function of goslings，40 1? day ?old Magang goslings were randomly divided into control group， PAMK group，CTX group and PAMK+CTX group，10 in each group. After 3 days of feeding，400 mg/kg PAMK was added to the diet of control group and PAMK group. At 20 days of age，CTX group and PAMK + CTX group were injected with cyclophosphamide in leg muscle at a dose of 40 mg/kg·BW for three consecutive days，other groups were injected with 0.5 mL of normal saline. The liver index and tissue structure were detected on 24?day ?old. The resultsshowed that cyclophosphamide could cause liver enlargement，tissue structure damage，hepatocyte DNA damage and liver function disorder in goslings. After the intervention of PAMK，the gosling liver and gallbladder indexes returned to normal，and the serum AST and AST levels were significantly reduced. The activity of ALT（P<0.05），the contents of TNF ? α，IL ? 10 and IL ? 1β were significantly increased（P<0.05），and the DNA damage of hepatocytes was significantly reduced. Cyclophosphamide significantly inhibited the protein levels of TLR4，NF ? κB1，TRAF6 and IKKε in the TLR4/NF ? κB signaling pathway（P<0.05），and increased the protein level of IκBα（P<0.05）. After PAMK treatment，the protein level of IκBα was significantly decreased（P<0.05），and the protein level of TRAF6 was significantly increased （P<0.05）， indicating that PAMK could effectively relieve the inhibitory effect of cyclophosphamide on key genes of TLR4/NF?κB pathway.

Key words: Polysaccharide of Atractylodes macrocephala Koidz（PAMK）, gosling, liver, cyclophosphamide, immune function, TLR4