广东畜牧兽医科技 ›› 2022, Vol. 47 ›› Issue (1): 14-16.DOI: 10.19978/j.cnki.xmsy.2022.01.03

• 试验研究 • 上一篇    下一篇

猪传染性胃肠炎病毒卵黄抗体的研制

赵子君,张 抒,魏苏红,程怀灵,魏平华*   

  1. (广州格雷特生物科技有限公司,广东 广州 510730)
  • 出版日期:2022-07-18 发布日期:2022-02-18
  • 通讯作者: 魏平华
  • 基金资助:
    广东省重点领域研发计划资助(2019B020218004)

Study and preparation of transmissible gastroenteritis virus egg yolk antibody

Zhao Zijun,Zhang shu,Wei Suhong,Cheng Huailing,Wei Pinghua*   

  1. (Guangzhou Great Biological Technology Co.,Ltd.,Guangzhou,Guangdong,510730)
  • Online:2022-07-18 Published:2022-02-18

摘要: 针对猪传染性胃肠炎病尚无有效的治疗药物,本文研究开发抗猪传染性胃肠炎病 毒卵黄抗体,以期为该病的防治提供技术支持。用猪传染性胃肠炎灭活疫苗按程序免疫90~ 140日龄健康商品海兰褐蛋鸡,三免14天后收集高免蛋进行卵黄抗体提取、ELISA抗体效价检 测及攻毒保护试验。高免蛋三免后14天效价可达到1∶256,并维持3个月,三免后120天效价 降至1∶128。对1日龄三元杂交仔猪的攻毒保护试验结果表明,未免疫卵黄抗体健康仔猪的发 病率、死亡率均达100%;而卵黄抗体免疫组的死亡率为0%、保护率达100%,表明该卵黄抗体 能够有效保护猪抵抗传染性胃肠炎病毒的攻击。

关键词: 猪传染性胃肠炎; 卵黄抗体; 防治

Abstract: There is no effective treatment for the transmissible gastroenteritis of pigs,so this paper investigates the development of transmissible gastroenteritis virus yolk antibody to provide technical support for the prevention and treatment of the disease. The inactivated vaccine of ransmissible gastroenteritis virus was used to immunize healthy commercial hens of hy ?line variety brown at the age of 90?140 day,14 days after the third immunization,the yolk antibody was extracted from the eggs of highly immunized chickens,ELISA antibody titer detection and attack protection test. The results showed that the antibody titer can reach 1∶256,and maintained for 3 months. 120 days after the third immunization,the antibody titer drops to 1∶128. The results of the attack protection test on 1?day?old three ?way crossbred piglets showed that the morbidity and mortality rate of healthy piglets not immunized with yolk antibody reached 100% ,while the mortality rate of the yolk antibody immune group is 0 and the protection rate reached 100% ,indicating that the yolk antibody can effectively protect piglets against the attack of the virus of transmissible gastroenteritis of swine.

Key words: the transmissible gastroenteritis of pigs; egg yolk antibody; prevention

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