关键词: 猪伪狂犬病病毒（PRV）； 分离鉴定； 致病性； gE基因； GD株

Abstract: A virus suspected to be porcine pseudorabies virus（PRV）was isolated from dead pigs in Guangdong province. The typical cytopathic effect（CPE）could be observed when the isolated virus was cultured on the swine testicular cells and it’s tissue culture infectious dose（TCID50）was 106.8/0.1 mL. A specific 370 bp long DNA fragment could be expanded using polymerase chain reaction（PCR）analysis from the isolated virus DNA. The sequence analysis results showed that the nucleotide identity of gE ? gene of PRV GD strain were between 97.6% ～99.9% comparing with several domestic and foreign PRV strains published in GenBank. The isolated virus strain was confirmed to be porcine pseudorabies virus and named as PRV GD strain. Inoculation of mice、rabbits、piglets and fattening pigs can cause the disease and death with strong pathogenicity.

Key words: pseudorabies virus（PRV）； isolation and identification； pathogenicity； gE?gene； GD strain