广东畜牧兽医科技 ›› 2023, Vol. 48 ›› Issue (5): 80-84.DOI: 10.19978/j.cnki.xmsy.2023.05.14

• • 上一篇    

不同禽白血病抗原检测试剂盒检测效果比较

刘洋1 ,陈顺艳2 ,王占新1 ,廖秋生2 ,吴志强1*   

  1. (1.温氏食品集团股份有限公司,广东 新兴 527400; 2.广东温氏南方家禽育种有限公司,广东 新兴 527400)
  • 出版日期:2023-10-18 发布日期:2023-10-18
  • 通讯作者: 吴志强

Comparing Detection Effects of Several Domestic Avian Leukosis Antigen Test Kits

LIU Yang1 ,CHEN Shunyan2 ,WANG Zhanxin1 ,LIAO Qiusheng2 ,WU Zhiqiang1**   

  1. (1.Wens Foodstuff Group Co.,Ltd.,Xinxing Guangdong 527400; 2.Guangdong Wens southern poultry Breeding Co.,Ltd.,Xinxing Guangdong 527400)
  • Online:2023-10-18 Published:2023-10-18

摘要: 禽白血病目前暂无有效的药物和疫苗进行防控,只能通过不断检测、淘汰阳性鸡以 净化种鸡群,在较短时间内最大程度筛检出阳性个体非常重要。因此,选择灵敏度高、特异性 强、稳定性好的抗原检测试剂盒很关键。受新冠疫情等因素的影响,某些进口的禽白血病病毒 抗原检测试剂盒出现供货紧张的局面,寻找检测灵敏度和批次间稳定性好的国产试剂盒十分 必要。因此,该研究通过对比评估多个国产抗原检测试剂盒(B、C、D)与某品牌进口禽白血病 病毒抗原试剂盒(A)对多个不同亚群禽白血病病毒的检测灵敏度和批次间稳定性,以期筛选出 检测灵敏度高、稳定性好的国产试剂盒,以保证净化工作能够持续进行。结果表明:不同试剂 盒对分离细胞上清液病毒的检测灵敏度和批次间稳定性存在一定差异,A、B、C 、D这四种试剂 盒的阳性率由高到低依次是B>D>C=A,其中以B试剂盒的检测灵敏度最高,试剂盒C的批次 间稳定性最好、与进口试验盒A的检测结果最接近。本研究为规模化种禽场禽白血病净化过程 中试剂盒的选择提供参考。

关键词: 禽白血病; 抗原检测试剂盒

Abstract: At present,there is no effective drug and vaccine to control the avian leukosis,so it is very important to detect and eliminate the positive individuals to purify the breeding flocks in a short time. Therefore,it is significant to select antigen detection kits with strong specificity,high sensitivity and good stability. Due to the impact of the epidemic novel coronavirus,some foreign antigen test kits can not be imported into China,it is necessary to find domestically?made kits with great sensitivity and stability. Therefore,in order to guarantee the continuous purification work,it is necessary to look for the domestic kits with great sensitivity and stability that is closest to the imported kit. The results showed that the sensitivity and stability of the different kits for the detection of the cell supernatant were different,the positive detection rate of the four kits was B>D>C=A. Among the four Avian Leukosis antigen test kits, the kit B had the highest detection sensitivity,the kit C had the best stability and closest to the imported kits. This study provides reference and data support for the kit selection in the process of Avian Leukosis purification.

Key words: Avian leukosis; Comparing detection; Antigen detection kits,domestically?made

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