关键词: 复方清肝汤； 犬； 急性肝损伤； 细胞焦亡

Abstract: （Objective） The purpose of this study was to explore the therapeutic effect and mechanism ofnamely the control group，the model group and the Chinese medicine treatment group. Acute liver injury model was established by intraperitoneal injection of 1 mg/kg of carbon tetrachloride，and the treatment group was given 1 ml/kg of Compound Qinggan Decoction every day for 14 days. At the end of the experiment，blood samples were collected for liver function test and liver tissue samples were taken out. H&E staining was used to observe the pathological changes of liver tissue，qRT ? PCR was used to detect the mRNA expression levels of hepatocyte pyroptosis ? related genes NLRP3，ASC，GSDMD，Caspase ? 1，IL ? 1β and IL ? 18，Western Blot was used to detect the expression levels of hepatocyte pyroptosis ? related proteins NLRP3，GSDMD，Caspase ?1，IL ?1β and IL ?18，and immunohistochemical method was used to detect the localization and expression of pyroptosis executive protein GSDMD.（Results）The experimental results showed that the activities of AST，ALT and γ?GT in the model group increased significantly（P< 0.05）；the arrangement of hepatocytes is disordered，and hepatocyte necrosis and inflammatory cell infiltration occur； the expression of pyroptosis?related genes NLRP3，ASC，IL?1β and IL?18 increased significantly（P<0.05），and the expression of pyroptosis?related proteins NLRP3，GSDMD，Caspase?1，IL?1β and IL?18 increased. After treatment with Compound Qinggan Decoction，the activities of AST，ALT and γ ? GT decreased clearly（P<0.05），and the pathological damage of liver tissue improved obviously. The mRNA expression levels of pyroptosis ? related genes NLRP3，ASC and IL ? 18 decreased evidently（P<0.05），and the expression levels of pyroptosis ? related proteins NLRP3，GSDMD，Caspase?1，IL?1β and IL?18 decreased.（Conclusion）Compound Qinggan Decoction can alleviate acute liver injury induced by carbon tetrachloride in canines by inhibiting the pyroptosis of hepatocytes. Compound Qinggan Decoction on acute liver injury induced by carbon tetrachloride in canines.（Methods）Eighteen mongrel canines aged from 12 to 15 months were randomly divided into three groups，with 6 canines in each group，

Key words: Compound Qinggan Decoction； Canine； Acute liver injury； Pyroptosis