关键词: 猪鼻支原体； 液体培养法； qPCR法； 检测； 灵敏度

Abstract: To Compare the detection sensitivity between liquid culture and real ??time qPCR for the samples collected at different growth phases of Mycoplasma hyorhinis（included in the Chinese Pharmacopoeia but very time?? consuming）（not included in the Chinese Pharmacopoeia but rapid），samples were collected at three different growth phases of Mycoplasma hyorhinis ：log phase（48 h），stationary phase（96 h）and dead phase（144 h）. Then the samples were diluted 10 times serially，and the detection sensitivity was tested by liquid culture and qPCR. The results showed that the detection sensitivity of liquid culture was about 100 times higher than that of the qPCR method in the log phase and stationary phase with a high proportion of viable mycoplasma. In the dead phase when theproportion of dead mycoplasma increased significantly，the detection sensitivity of qPCR was about 1000 times higher than that of liquid culture. When the log phase sample was concentrated（about 100 times）by centrifugation，they were detected again by qPCR. Compared with the sample before concentration，the detection sensitivity of the sample was increased by 100 times. In conclusion，the main factors affecting the relative detection sensitivity of liquid culture and qPCR are the sample addition volumes in their respective systems and the proportion of viable and dead mycoplasma cells in the samples. The difference in sensitivity between the two methods caused by the former factor can be reduced by high??speed centrifugal concentration，so that the sensitivity of the two methods is the same.

Key words: Mycoplasma hyorhinis； Liquid culture； qPCR； Detection sensitivity