关键词: 反向遗传学技术； 新城疫病毒载体； 疫苗

Abstract: Newcastle Disease Virus（NDV）has been successfully used as a vaccine carrier in a variety of human and animal diseases to develop vaccines. Studies have shown that recombinant Newcastle disease virus (rNDV) vector vaccines have better performance in humoral immunity，mucosal immunity，and challenge protection compared with traditional vaccines. NDV vector has become a ideal tool for the development of safe，efficient，low toxicity，and better immunogenicity vector vaccines. The advances of novel vaccines for poultry，mammals，and humans using NDV as a vaccine vector are reviewed，to provide new ideas for the development of more epidemic vaccines using NDV vector.

Key words: Reverse genetics； Newcastle disease virus vector； Vaccines