广东畜牧兽医科技 ›› 2023, Vol. 48 ›› Issue (1): 57-62.DOI: 10.19978/j.cnki.xmsy.2023.01.11

• 专题综述 • 上一篇    下一篇

广东历年PRRSV流行毒株与疫苗株的重组分析

康溥1,4 ,陈强2 ,林牧3 ,王晓虎1 ,王刚1 ,吕宗吉4* ,陈晶1*   

  1. (1.广东省农业科学院动物卫生研究所/广东省畜禽疫病防治研究重点实验室 农业农村部兽用药物与诊断技术广东科学观测实验站,广东 广州 510640; 2.广西壮族自治区贵港市桂平市动物检疫站,广西 贵港 537200; 3.广东省阳江市阳东区动物卫生监督所,广东 阳江 529900; 4.佛山科学技术学院,广东 佛山 528000)
  • 出版日期:2023-02-18 发布日期:2023-02-18
  • 通讯作者: 陈晶,吕宗吉
  • 基金资助:
    广东省自然科学基金项目(2020A1515011112);广东省非洲猪瘟科技应急防控专题项目(20190211)

Recombination analysis of PRRSV epidemic strains and vaccine strains over the years in Guangdong

KANG Pu1 ,CHEN Qiang 2 ,LIN Mu3 ,WANG Xiaohu1 ,WANG Gang1 ,LU ZongJi4* ,CHEN Jing1*   

  1. (1. Institute of Animal Health,Guangdong Academy of Agricultural Sciences,Key Laboratory of Livestock Disease Prevention of Guangdong Province,Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province,Ministry of Agriculture,P.R.China,Guangzhou Guangdong 510640; 2.Guiping animal quarantine station,Guigang City,Guangxi Zhuang Autonomous Region,Guigang Guangxi 537200; 3.Yangdong District Animal Health Supervision Institute,Yangjiang,Yangjiang Guangdong 529900; 4.Foshan University,Foshan Guangdong 528000)
  • Online:2023-02-18 Published:2023-02-18

摘要: 从GenBank上可查的全部424株猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)全基因序列中筛选出了42株来源于广东的PRRSV毒株全基 因组序列与6株PRRSV疫苗株全基因序列,用RDP4(Recombination detecting program 4,RDP4) 软件进行重组分析。发现广东株之间的重组事件集中在2010年、2011年和2015年。JXA1?R? CN?2009作为亲本毒株出现在12个重组事件中,重组体的年份从2010年延续到2018年。疫苗 株Ingelvac ATP?US?2006与CH?1R?CN?2008作为次要亲本毒株出现在事件10中,该事件的重组 体为广东2018年的毒株。在事件18和25中可以见到疫苗株之间的重组,但软件同时显示该 事件可能是自然进化而非重组。该研究对广东猪场科学规范的使用弱毒疫苗有着一定指导意 义。

关键词: 广东PRRSV毒株; 疫苗株; 重组分析

Abstract: The complete genome sequences of 42 PRRSV strains derived from Guangdong and completerecombination events occurred in the parental strain of JXA1?R?CN?2009 from 2010 to 2018. Vaccine strains Ingelvac ATP?US?2006 and CH?1R?CN?2008 appeared as secondary parental strains in event 10,the recombinant strain of this event was Guangdong strain isolated from 2018. Recombination between vaccine strains can be seen in events 18,25, but the software also shows that this event may have evolved naturally rather than recombined. This paper has certain significance for the scientific and standardized use of modified live vaccines in Guangdong pig farms. sequences of 6 PRRSV vaccine strains were selected from all 424 PRRSV complete sequences available on GenBank database to carry out recombination analysis using RDP4(Recombination detecting program 4,RDP4) software. Recombination events between Guangdong strains were found to be concentrated in 2010,2011 and 2015. 12

Key words: Guangdong PRRSV strain; Vaccine strain; Recombination analysis

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