关键词: ELISA检测； 兽医实验室； 问题； 探讨

Abstract: The detection of serum antibodies in the veterinary laboratory mainly adopts the ELISA detection method. This paper discusses the common problems in the daily ELISA test based on the actual operation summary of the veterinary laboratory. It is found that the influencing factors of the common problems in the ELISA test include sample processing，experimental operation methods，experimental equipment，and so on. It is suggested that the ELISA operation method must be strictly mastered in veterinary laboratory testing to reduce or avoid experimental failures caused by operational errors. This article can provide help for veterinary laboratory colleagues.

Key words: ELISA detection； Veterinary laboratory； Problem； Discussion