关键词: 鸡蛋, 喹诺酮类药物, 同位素内标法, 高效液相色谱?串联质谱

Abstract: A high performance liquid chromatography ?tandem mass spectrometry method was developed for the simultaneous determination of 14 quinolones residues in eggs. The samples were extracted with EDTA ? Mcllvaine buffer，followed by defatted with n?hexane，and purified by HLB solid phase extraction column，separated by reversed? phase C 18 column，determined by multiple reaction monitoring positive ion mode，and quantified by isotope internal standard method. Under the optimized conditions，the detection limit and quantification limit of 14 quinolones were 0.5 μg/kg and 2 μg/kg，respectively. The linearity was fine in the range of 2~100 ng/mL，and the correlation coefficients r was greater than 0.99. Adding 2，5，and 10 μg/kg to blank eggs sample，the average recoveries were 83.9%~110.7%，the intra ?assay coefficient of variation were between 1.5% and 13.7%（n=6），and the inter ?assay coefficient of variation were from 4.5% to 13.1%（n=18）. The results showed that the method was sensitive，accurate， and reproducible，and was suitable for the qualitative and quantitative detection of quinolones residues in eggs.

Key words: Eggs, Quinolones, Isotopic internal standard method, High performance liquid chromatography?tandem mass spectrometry（HPLC?MS/MS）