广东畜牧兽医科技 ›› 2022, Vol. 47 ›› Issue (2): 43-50.DOI: 10.19978/j.cnki.xmsy.2022.02.09

• 专题综述 • 上一篇    

新型冠状病毒检测方法研究进展

蔡琳琳,梁健鹏,陈礼斌,向斌,任涛*   

  1. (华南农业大学兽医学院、华南农业大学兽医学院、农业农村部兽用疫苗创制重点实验室、 人兽共患病防控制剂国家地方联合工程实验室、广东省动物源性人兽共患病预防 与控制重点实验室,广东 广州 510640)
  • 出版日期:2022-04-18 发布日期:2022-04-18
  • 通讯作者: 任涛
  • 基金资助:
    2020佛山市“新型冠状病毒感染的肺炎”应急科技攻关专项(NO.2020001000151)

Research progress in test technique for severe acute respiratory syndrome coronavirus 2

CAI Linlin,LIANG Jianpeng,CHEN Libin,XIANG Bin,REN Tao*   

  1. (Veterinary medicine college of South China Agricultural University,Guangdong,guangzhou 510640)
  • Online:2022-04-18 Published:2022-04-18

摘要: 新型冠状病毒(Severe acute respiratory syndrome coronavirus 2,SARS?CoV?2)传播能 力强,易感动物种类多。目前,已出现多种变异株,可能存在逃脱现有疫苗保护的风险。认识 人与动物界及其与疾病传播的相关性仍然是许多新兴的人畜共患病的关键控制点。本文简述 了SARS?CoV?2的结构和突变毒株类型,并阐述了目前针对SARS?CoV?2的一系列检测手段的 技术原理、特点,以及其应用现状,总结各方法的优缺点,最后提出完善常规疫病监测系统的 建议,阐明对畜牧生物安全防控的重要意义。

关键词: SARS?CoV?2; COVID?19; qRT?PCR; IAT; 免疫检测; 生物安全

Abstract: There are many kinds of susceptible animals for severe acute respiratory syndrome coronavirus 2 (SARS?CoV?2),which has a strong transmission capacity. At this stage,a variety of mutants of SARS?CoV?2 have appeared,which may be at risk of evading the protection of existing vaccines. Understanding the human ? animal interface and its relevance to disease transmission remains a critical control point for many emerging zoonoses. In this paper,the structure and mutant strains of SARS ? CoV ? 2 were briefly described. Further,the technical principles, characteristics and application status of a series of detection methods for SARS?CoV?2 were described. Meanwhile,the advantages and disadvantages of each method were summarized in this paper. And finally,some suggestions were put forward to improve the routine disease monitoring system,after that,the importance of livestock husbandry biosecurity prevention and control were clarified step by step.

Key words: SARS?CoV?2; COVID?19; qRT?PCR; IAT; Immunodetection; Biosecurity

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